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RNA Polymerase II Antibody [CTD4H8]

Additional information

Catalog Number: 5430-MSM1
Reactivity:

, ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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Catalog Number Formulation Size Price
5430-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5430-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5430-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
RNA Polymerase II Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RNA Polymerase II / Poll II Mouse Monoclonal Antibody (CTD4H8).

RNA Polymerase II Antibody - Image 2

SDS-PAGE Analysis of Purified RNA Poll II Mouse Monoclonal Antibody (CTD4H8). Confirmation of Purity and Integrity of Antibody.

RNA Polymerase II Antibody - Image 3

Western Blot Analysis of Human HAP1, K562, PC3, HePG2 & NIH3T3 cell lysates using RNA Poll II Mouse Monoclonal Antibody (CTD4H8).

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Rat
Clone
CTD4H8
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Chromosome, Cytoplasm, Nucleus
Gene Name
POLR2A
Positive Control
HAP1, HePG2, K562, NIH3T3 cells. Human testis., PC3
Immunogen
Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).
  • (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.
Key References
  • Madak-Erdogan, Z., et al. 2008. Mol. Endocrinol. 22: 2116-2127.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5430
SwissProt
P24928
Chromosome Location
17p13.1
Mol. Weight of Antigen
192-253kDa
Alternate Names
DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit; DNA-directed RNA polymerase II subunit RPB1; hRPB220; hsRPB1; POLR2; Polr2a; POLRA; Polymerase (RNA) II (DNA directed) polypeptide A 220kDa; RNA polymerase II subunit B1; RPBh1; RpIILS; RPO2; RPOL2

Summary

RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

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