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Home >> Antibodies >> Protein Kinase C iota / lambda / PRKCI

Protein Kinase C iota / lambda / PRKCI

Mouse Monoclonal Antibody [Clone PRKCI/4912]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human brain stained with Protein Kinase C iota / lambda Mouse Monoclonal Antibody (PRKCI/4912).

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SDS-PAGE Analysis Purified PRKCI Mouse Monoclonal Antibody (PRKCI/4912). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Protein Kinase C iota / lambda Mouse Monoclonal Antibody (PRKCI/4912).

Product Details

Synonyms

aPKC lambda/iota; Atypical protein kinase C lambda/iota; Atypical protein kinase C-lambda/iota; nPKC-iota; nPKCiota; PKC lambda/iota; PKCI; PKCiota; PKClambda; PRKC iota; PRKC lambda/iota; PRKCI; Protein kinase C iota type

Positive Control

Brain, liver or placenta.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa100-300) of human PRKCI protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Cell Membrane. Nucleus.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

68kDa

Specificity & Comments

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (, I, II and ) and novel (n) PKC isoforms ( L, , , , , l/i, m and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC L and are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Bicaku, E., et al. 2005. Cyclin-dependent kinase activating kinase/Cdk7 co-localizes with PKC-i in human glioma cells. Tissue Cell 37: 53-58.

Bioinformatics

  •  Entrez Gene ID: 5584
  •  Gene Symbol: PRKCI
  •  SwissProt: P41743
  •  Chromosome Location: 3q26.2
  •  Unigene: 478199