Home >> Antibodies >> Protein Kinase C α / PRKCA

Protein Kinase C α / PRKCA

Mouse Monoclonal Antibody [Clone 133]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with Protein Kinase C ’ Mouse Monoclonal Antibody (133).

Formalin-fixed, paraffin-embedded human tonsil stained with Protein Kinase C ’ Mouse Monoclonal Antibody (133).

Formalin-fixed, paraffin-embedded human tonsil stained with Protein Kinase C ’ Mouse Monoclonal Antibody (133).

Formalin-fixed, paraffin-embedded human tonsil stained with Protein Kinase C ’ Mouse Monoclonal Antibody (133).

Product Details

Synonyms

PKC alpha; PKC-A; PKC-alpha; PKCA; PRKCA; Protein kinase C alpha; Protein kinase C alpha type

Positive Control

Normal human kidney or tonsil tissue. HEK293 whole cell lysates.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCα.

Cellular Localization

Cytoplasm. Cell Membrane. Nucleus.

Species Reactivity

Human, Rat and Mouse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

77kDa

Specificity & Comments

Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.