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Home >> Antibodies >> Proliferation Marker

Proliferation Marker

Mouse Monoclonal Antibody [Clone JC1]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).

img

Formalin-fixed, paraffin-embedded human Lymph Node stained with Proliferation Marker Mouse Monoclonal Antibody (JC1).

Product Details

Positive Control

HeLa cells (IF). Human lymph node (IHC).

Known Applications & Suggested Dilutions

Western Blot, IF, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Pokeweed-stimulated lymphocytes

Cellular Localization

Nucleus

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

212kDa and 123kDa

Specificity & Comments

JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Rose, DS et al. J Clin Pathol. 1994 Nov; 47(11): 1010-1014.
  2. Wang et al. 2016. Am J Cancer Res. 6(6):1331-44.

Bioinformatics

NBT03329