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Home >> Antibodies >> POLE3 / CHRAC17

POLE3 / CHRAC17

Mouse Monoclonal Antibody [Clone PCRP-POLE3-2F10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Flow Cytometric Analysis of PFA-fixed K562 cells. POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

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Formalin-fixed, paraffin-embedded human lymphoma stained with POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-2F10) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis. Purified POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-2F10). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-2F10).

Product Details

Synonyms

Arsenic transactivated protein; ASTP; CHRAC17; Chromatin accessibility complex 17 kDa protein; DNA polymerase II subunit 3; Histone fold protein CHRAC17; HuCHRAC17; Polymerase (DNA directed) epsilon 3 (POLE3); YBL1

Positive Control

HeLa or K562 cells.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human POLE3 protein

Cellular Localization

Nucleus. Cytoplasm.

Species Reactivity

Human. Predicted to react in Mouse, Rat and Xenopus.

Host / Ig Isotype

Mouse / IgG1

Mol. Weight of Antigen

16.86kDa

Specificity & Comments

DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  2. Nucleic Acids Res. 38:D142-D148 (2010).

Bioinformatics

  •  Entrez Gene ID: 54107
  •  Gene Symbol: POLE3
  •  SwissProt: Q9NRF9
  •  Chromosome Location: 9q32
  •  Unigene: 108112