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POLE3 / CHRAC17 Antibody [PCRP-POLE3-2F10]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
54107-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
54107-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
54107-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 /
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa or K562 cells.
Immunogen
Recombinant full-length human POLE3 protein
Alternate Names
DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, Arsenic transactivated protein; ASTP; CHRAC17; Chromatin accessibility complex 17 kDa protein; DNA polymerase II subunit 3; Histone fold protein CHRAC17; HuCHRAC17; Polymerase (DNA directed) epsilon 3 (POLE3); YBL1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-POLE3-2F10
Chromosome Location
9q32
Mol. Weight of Antigen
16.86kDa

Functions

  • Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed:10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed:14759371).

Key References

  • Blackshaw S., et al. Nature methods15.5 (2018 May): 330-338.
  • Nucleic Acids Res. 38:D142-D148 (2010).

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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