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Home >> Antibodies >> p53 Tumor Suppressor Protein

p53 Tumor Suppressor Protein

Mouse Monoclonal Antibody [Clone SPM349]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00

Product Details

Synonyms

Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Positive Control

MDA-MB-231 or A431 cells. Breast or Colon carcinoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Frozen only) (1-2ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Immunogen

Gel-Purified p53-beta-galactosidase fusion protein containing murine p53 from aa 14-389

Cellular Localization

Nuclear

Species Reactivity

Human. Monkey. Cow. Dog. Mouse. Rat. Hamster.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

53kDa

Specificity & Comments

The specificity of this monoclonal antibody to its intended target was tested by HuProtTM Array, containing more than 19,000, full-length human proteins. SPM349 binds to the C-terminus (aa213-217) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 Binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. p53 Is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Gannon et al. 1990. EMBO J. 9(5):1595-602.
  2. Bartek et al. 1991. Oncogene. 6(9):1699-703.

Bioinformatics

  •  Entrez Gene ID: 7157
  •  Gene Symbol: TP53
  •  SwissProt: P04637
  •  Chromosome Location: 17p13.1
  •  Unigene: 654481
NBT01634