Home >> Antibodies >> OCT-4 (POU5F1) (Germ Cell Tumor Marker)

OCT-4 (POU5F1) (Germ Cell Tumor Marker)

Recombinant Rabbit Monoclonal Antibody [Clone OCT4/6875R]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

OCT3; OCT3A; OCT4; OCT4A; OCT-3/4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Positive Control

Raji or U-937 cells. Human seminoma or testicular carcinoma

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)

Cellular Localization

Nucleus

Species Reactivity

Human

Host / Ig Isotype

Rabbit / IgG, kappa

Mol. Weight of Antigen

38.6kDa

Specificity & Comments

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.