Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

OCT-4 (POU5F1) (Germ Cell Tumor Marker) [OCT4/6875R]

Name: OCT-4 (POU5F1) (Germ Cell Tumor Marker)
SKU: 5460-RBM5
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

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Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Isotype

IgG

Cellular Localization

Cytoplasm, Nucleus

Gene Name

POU5F1

Mol. Weight of Antigen

38.6kDa

Immunogen

Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)

Database Links

Entrez Gene ID

5460

Unigene

249184

SwissProt

Q01860

Additional Information

Clone

OCT4/6875R

Human Gene Symbol

POU5F1

Chromosome Location

6p21.33

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.