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Home >> Antibodies >> Nucleoside Diphosphate Kinase A / nm23-H1

Nucleoside Diphosphate Kinase A / nm23-H1

Mouse Monoclonal Antibody [Clone NME1/2738]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Western Blot Analysis of A549, PC3, K562 cell lysates using NME1 / nm23-H1 Mouse Monoclonal Antibody (NME1/2738).

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Formalin-fixed, paraffin-embedded human breast carcinoma stained with NME1 / nm23-H1 Mouse Monoclonal Antibody (NME1/2738).

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Formalin-fixed, paraffin-embedded human cervix stained with NME1 / nm23-H1 Mouse Monoclonal Antibody (NME1/2738).

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SDS-PAGE Analysis Purified NME1 / nm23-H1 Mouse Monoclonal Antibody (NME1/2738). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific to NME1 / nm23-H1 Mouse Monoclonal Antibody (NME1/2738).

Product Details

Synonyms

AWD; Granzyme A activated DNase (GAAD); GZMA activated DNase; Metastasis inhibition factor NM23; NBS; NDKA; NM23; nm23-H1; NM23-M1; NM23H1B, included; NME/NM23 nucleoside diphosphate kinase 1; Nme1; NME1-NME2 spliced read-through transcript, included; Non-metastatic cells 1, protein (NM23A) expressed in; Nonmetastatic cells 1, protein expressed in; Nonmetastatic protein 23; Tumor metastatic process-associated protein

Positive Control

HeLa, A549 or Jurkat cells. Ubiquitously expressed in all tissues.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human NME1 protein

Cellular Localization

Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

17kDa

Specificity & Comments

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tong, Y., et al. 2015. Cancer Lett. 361: 207-217.

Bioinformatics

  •  Entrez Gene ID: 4830
  •  Gene Symbol: NME1
  •  SwissProt: P15531
  •  Chromosome Location: 17q21.33
  •  Unigene: 463456