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NME1 / nm23-H1 / NDPK-A (Suppressor of Metastasis) Antibody [CPTC-NME1-2]

In Stock
Catalog Number Formulation Size Price
4830-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4830-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4830-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Western Blot (WB)
2-4ug/ml

Summary

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP-K). In humans, NDP kinase A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human C-Myc promotor.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
Brain, HepG2 or HeLa whole cell lysates. Human heart, placenta and lung carcinoma.
Immunogen
Recombinant full-length human NME1 protein
Alternate Names
Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, AWD; GAAD; Granzyme A activated DNase; Metastasis inhibition factor NM23; NB; NBS; NDP kinase A; NM23 long variant, included; NM23H1B; NME/NM23 nucleoside diphosphate kinase 1; NME1-NME2 spliced read-through transcript, included; Non-metastatic protein 23, homolog 1; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
CPTC-NME1-2
Chromosome Location
17q21.33
Mol. Weight of Antigen
23kDa

Functions

  • Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Key References

  • Steeg, P.S., et al. 1988. J. Natl. Cancer Inst. 80: 200-209

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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