2 Union Square, Union City, CA 94587
contact@NeoBiotechnologies.com
510-376-5603

Home >> Antibodies >> MyoD1 (Rhabdomyosarcoma Marker)

MyoD1 (Rhabdomyosarcoma Marker)

Mouse Monoclonal Antibody [Clone SPM427]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
img

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (SPM427).

Product Details

Synonyms

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Positive Control

Rhabdomyosarcoma.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189.

Cellular Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Species Reactivity

Human. Mouse. Rat. Chicken.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

45kDa

Specificity & Comments

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm's tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing's sarcomas and alveolar soft part sarcomas.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.
  2. Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
  3. Parham DM et. al. Acta Neuropathologica, 1994, 87:605-11.

Bioinformatics

  •  Entrez Gene ID: 4654
  •  Gene Symbol: MYOD1
  •  SwissProt: P15172
  •  Chromosome Location: 11p15.1
  •  Unigene: 181768
NBT00191