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Recombinant MyoD1 (Rhabdomyosarcoma Marker) Antibody [rMYOD1/6911]

In Stock
Catalog Number Formulation Size Price
4654-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4654-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4654-MSM11-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase, thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS). RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors. Studies suggest, anti-MyoD1 may be used together with anti-Myogenin and anti-Desmin as a panel of markers since any RMS is virtually never negative for all three markers simultaneously.

Product Properties & Targets

Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
Human fetal skeletal muscle or rhabdomyosarcoma.
Immunogen
Recombinant fragment (around aa1-100) of human MyoD1 protein (exact sequence is proprietary)
Alternate Names
Myoblast determination protein 1, Class C basic helix-loop-helix protein 1, Myogenic factor 3, bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rMYOD1/6911
Chromosome Location
11p15.1
Mol. Weight of Antigen
45kDa

Functions

  • Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).

Key References

  • Thulasi R et. al. Cell Growth and Differentiation, 1996, 7(4):531-41.
  • Wesche WA et. al. American Journal of Surgical Pathology, 1995, 19(3):261-9.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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