Home >> Antibodies >> MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rMUC1/4418]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 / EMA Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

SDS-PAGE Analysis Purified MUC-1 Recombinant Mouse Monoclonal Antibody (rMUC1/4418). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of MCF-7 cell lysate using MUC-1 / EMA Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC-1 / EMA Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

SDS-PAGE Analysis Purified MUC-1 Recombinant Mouse Monoclonal Antibody (rMUC1/4418). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of MCF-7 cell lysate using MUC-1 / EMA Recombinant Mouse Monoclonal Antibody (rMUC1/4418).

Product Details

Synonyms

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, H23AG, KL-6, MAM6, MUC-1/SEC, MUC-1/X, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen

Positive Control

MCF-7 or MDA-231 cells. Human breast, colon, ovarian or endometrial carcinoma.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human MUC1 protein

Cellular Localization

Cytoplasm. Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

265-400kDa

Specificity & Comments

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and colorectal carcinoma. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms such as schwannomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.