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Home >> Antibodies >> Interleukin-1beta (IL-1beta)

Interleukin-1beta (IL-1beta)

Mouse Monoclonal Antibody [Clone IL1B/6687]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human adrenal gland stained with Interleukin-1 Beta (IL-1B) Mouse Monoclonal Antibody (IL1B/6687)at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; IL-1 beta; IL1B; IL1F2; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; Osteoclast activating factor (OAF); Preinterleukin 1 beta; Pro interleukin 1 beta

Positive Control

LPS stimulated human peripheral blood mononuclear cells. Human colon or liver tissue.

Known Applications & Suggested Dilutions

ELISA, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human IL1B protein (exact sequence is proprietary)

Cellular Localization

Cytoplasm. Extracellular (secreted).

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

31kDa (proprotein); 17kDa (mature)

Specificity & Comments

Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of MAb IL1B/463 and IL1B/6687 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Dumoulin FL, et al. Intrahepatic expression of interleukin-1beta and tumor necrosis factor-alpha in chronic hepatitis C. J Infect Dis. 1999 Nov;180(5):1704-8.

Bioinformatics

  •  Entrez Gene ID: 3553
  •  Gene Symbol: IL1B
  •  SwissProt: P01584
  •  Chromosome Location: 2q14.1
  •  Unigene: 126256