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  • Drug Targets, HuProt™ Validated

Interleukin-1beta (IL-1beta) Antibody [IL1B/3993]

Additional information

Catalog Number: 3553-MSM6
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3553-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3553-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3553-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Interleukin-1beta (IL-1beta) Antibody

Formalin-fixed, paraffin-embedded human colon stained with Interleukin-1 Beta (IL-1B) Mouse Monoclonal Antibody (IL1B/3993).

Interleukin-1beta (IL-1beta) Antibody - Image 2

Formalin-fixed, paraffin-embedded human adrenal gland stained with Interleukin-1 Beta (IL-1B) Mouse Monoclonal Antibody (IL1B/3993).

Interleukin-1beta (IL-1beta) Antibody - Image 3

Formalin-fixed, paraffin-embedded human adrenal gland stained with Interleukin-1 Beta (IL-1B) Mouse Monoclonal Antibody (IL1B/3993).

Human Interleukin-1 beta Antibody in SDS-PAGE

Human Interleukin-1 beta Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified IL-1B Mouse Monoclonal Antibody(IL1B/3993). Confirmation of Purity and Integrity of Antibody.

  • ELISA
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Clone
IL1B/3993
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytosol, Extracellular exosome, Lysosome, Secreted
Gene Name
IL1B
Positive Control
LPS stimulated human peripheral blood mononuclear cells. Human colon or liver tissue.
Immunogen
Recombinant fragment of human IL1B protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Reactivity:

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Additional information

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Host:

Reactivity:

, , ,

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Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Potent pro-inflammatory cytokine (PubMed:3920526, PubMed:10653850, PubMed:12794819). Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production (PubMed:3920526). Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6 (PubMed:12794819). Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33377178, PubMed:33883744).
Key References
  • Dumoulin FL, et al. Intrahepatic expression of interleukin-1beta and tumor necrosis factor-alpha in chronic hepatitis C. J Infect Dis. 1999 Nov;180(5):1704-8.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3553
SwissProt
P01584
Chromosome Location
2q14.1
Mol. Weight of Antigen
31kDa (proprotein); 17kDa (mature)
Alternate Names
Interleukin-1 beta, Catabolin, Catabolin; Hematopoietin 1 (H1); IFN beta inducing factor; IL-1 beta; IL1B; IL1F2; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; Osteoclast activating factor (OAF); Preinterleukin 1 beta; Pro interleukin 1 beta

Summary

Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of MAb IL1B/463 and IL1B/3993 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

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