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Home >> Antibodies >> HLA-DQ (MHC II)

HLA-DQ (MHC II)

Recombinant Rabbit Monoclonal Antibody [Clone HLA-DQA1/2866R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified HLA-DQ Rabbit Recombinant Monoclonal Ab (HLA-DQA1/2866R). Confirmation of Purity and Integrity of Antibody.

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Flow Cytometric Analysis of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal Antibody (HLA-DQA1/2866R) followed by goat anti-Rabbit IgG-CF488 (Blue); Isotype Control (Red).

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Immunofluorescent staining of Raji cells. HLA-DQ Rabbit Recombinant Monoclonal (HLA-DQA1/2866R) followed by goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Product Details

Synonyms

CELIAC1; DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE); HLA class II histocompatibility antigen DQ(1) alpha chain; HLA class II histocompatibility antigen DQ(3) alpha chain; HLA DQA1; HLA DQB1; IDDM1; Leucocyte antigen DQA1; Major histocompatibility complex class II DQ alpha 1; Major histocompatibility complex class II DQ beta 1

Positive Control

Raji cells. Tonsil or lymph node.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen only) (1-2ug/ml for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HLA-DQ protein

Cellular Localization

Cell Surface

Species Reactivity

Human. Pig.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

60kDa

Specificity & Comments

Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

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200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Spits H; Keizer G; Borst J; Terhorst C; Hekman A; de Vries JE. Hybridoma, 1983, 2(4):423-37.

Bioinformatics

NBT02866