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HLA-DQ (MHC II) Antibody [SPV-L3]

Additional information

Catalog Number: 3117-MSM1
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3117-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3117-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3117-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
HLA-DQA Antibody in SDS-PAGE

HLA-DQA Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified HLA-DQA Mouse Monoclonal Antibody (SPV-L3). Confirmation of Purity and Integrity of Antibody.

HLA-DQ (MHC II) Antibody - Image 2

Flow Cytometric Analysis of Raji cells. HLA-DQ Mouse Monoclonal Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human, Pig
Clone
SPV-L3
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, trans-Golgi network membrane
Gene Name
HLA-DQA1
Positive Control
Raji cells. Tonsil or lymph node.
Immunogen
T4-positive CTL clone HG-38
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Key References
  • Spits H., Borst J., Giphan M., Coligan J., Terhorst C. and de Vries J., Eur. J. Immunol. 14, (1984).
  • Spits H; Keizer G; Borst J; Terhorst C; Hekman A; de Vries JE. Hybridoma, 1983, 2(4):423-37.
  • Bontrop R., Schreuder G., Mikalski E., van Miltenburg R. and Giphan M., Tissue Antigens, 27, (1986).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3117; 3118; 3119
SwissProt
P01908
Chromosome Location
6p21.3
Mol. Weight of Antigen
60kDa
Alternate Names
HLA class II histocompatibility antigen, DQ alpha 1 chain, DC-1 alpha chain, DC-alpha, HLA-DCA, MHC class II DQA1, CELIAC1; DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE); HLA Class II DQ; HLA class II histocompatibility antigen DQ; HLA class II histocompatibility antigen DQ(1) alpha chain; HLA class II histocompatibility antigen DQ(3) alpha chain; HLA DQA1; HLA DQB; HLA DQB1; IDDM1; Leucocyte antigen DQA1; Major histocompatibility complex class II DQ alpha 1; Major histocompatibility complex class II DQ beta 1; MHC class II antigen HLA DQ beta 1; MHC class II HLA DQ alpha 1; MHC class II HLA DQ beta glycoprotein; MHC DQ beta

Summary

Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

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