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Home >> Antibodies >> Histone H1 (Pan Nuclear Marker)

Histone H1 (Pan Nuclear Marker)

Recombinant Rabbit Monoclonal Antibody [Clone AE-4]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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Formalin-fixed, paraffin-embedded human Skin Basal Cell Carcinoma stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4).

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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4).

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SDS-PAGE Analysis Purified Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4). Confirmation of Purity and Integrity of Antibody.

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Western Blot Analysis of human Heart tissue lysate using Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4).

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Immunofluorescence staining of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (AE-4) followed by goat anti-mouse IgG-CF488 (green). Phalloiden is used to stain the membrane (Red).

Product Details

Synonyms

H1(0); H1.1; H1.2; H1.3; H1.4; H1.5; H10; H1A; H1F0; H1F1; H1F2; H1F3; H1F4; H1F5; H1FNT; H1FOO; H1FT; H1FV; H1FX; H1t; H1T2; H1X; HANP1; His1; HisC; HIST1; HIST1H1A; HIST1H1B; HIST1H1C; HIST1H1D; HIST1H1E; HIST1H1T; Oocyte-specific histone H1; Testicular H1 histone

Positive Control

HeLa, A-431, LNCap or Jurkat cells. Human pancreas or breast carcinoma tissues. Human heart tissue lysate.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Nuclei of human leukemia biopsy cells

Cellular Localization

Nuclear

Species Reactivity

Human. Mouse. Rat.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

~30kDa

Specificity & Comments

Please note that this antibody is a recombinant Rabbit version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a pure preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.

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200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Bioinformatics

  •  Entrez Gene ID: 3005
  •  Gene Symbol: H1
  •  SwissProt: Multiple
  •  Chromosome Location: 3q21-q22; 6q21-q22; 22q13.1
  •  Unigene: 226117, 97358
NBT03325