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Home >> Antibodies >> HGAL (Human Germinal Center Associated Lymphoma Marker)

HGAL (Human Germinal Center Associated Lymphoma Marker)

Mouse Monoclonal Antibody [Clone HGAL/2373]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human testis stained with HGAL Mouse Monoclonal Antibody (HGAL/2373).

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SDS-PAGE Analysis Purified HGAL Mouse Monoclonal Antibody (HGAL/2373). Confirmation of Purity and Integrity of Antibody.

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Analysis of Protein Array containing >19,000 full-length human proteins using HGAL Mouse Monoclonal Antibody (HGAL/2373).

Product Details

Synonyms

GCAT2; GCET2 germinal center expressed transcript 2; Germinal center associated lymphoma (hGAL); Germinal center B-cell-expressed transcript 2 protein; Germinal center-associated signaling and motility protein

Positive Control

Daudi, Raji or U266 cells. Human lymphocytes. Human lymph node, tonsil or testis.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (178 Amino acid residues from around aa1-200) of human HGAL protein

Cellular Localization

Cytoplasm. Cell membrane.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

21kDa

Specificity & Comments

Recognizes a protein of kDa, which is identified as HGAL. It contains a putative PDZ-interacting domain, an immunoreceptor tyrosine-based activation motif (ITAM), and two putative SH2 binding sites. In B cells, its expression is specifically induced by interleukin-4. HGAL is specifically expressed in germinal center B-cells, but is absent in mantle and marginal zone B-cells and in the inter-follicular and para-cortical regions in normal tonsils and lymph nodes. Its high degree of specificity for germinal center B-cells makes anti-HGAL an ideal marker for the detection of germinal center-derived B-cell lymphomas. HGAL expression has been used to help elucidate nodal marginal zone lymphoma (NMZL) from cases of diffuse follicle center lymphoma. Additionally, HGAL expression was shown to correlate with survival in patients with diffuse large B-cell lymphoma (DLBCL).

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Natkunam Y, et al. Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation. Blood. 2005 May 15;105(10):3979-86.

Bioinformatics

  •  Entrez Gene ID: 257144
  •  Gene Symbol: GCSAM
  •  SwissProt: Q8N6F7
  •  Chromosome Location: 3q13.2.2
  •  Unigene: 49614