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Home >> Antibodies >> GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)

Recombinant Rabbit Monoclonal Antibody [Clone HSP90B1/3168R]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified GRP94 Recombinant Rabbit Monoclonal (HSP90B1/3168R). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).

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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R).

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Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Recombinant Rabbit Monoclonal Antibody (HSP90B1/3168R) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).

Product Details

Synonyms

Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Positive Control

HepG2, U20S cells. About 50% of breast tumors express grp94 protein.

Known Applications & Suggested Dilutions

Flow Cyt, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human HSP90B1 protein

Cellular Localization

Cytoplasmic and nuclear

Species Reactivity

Human. Monkey. Horse. Cow. Sheep. Pig. Rabbit. Dog. Rat. Mouse. Hamster. Guinea Pig. Chicken. Xenopus laevis.

Host / Ig Isotype

Rabbit / IgG

Mol. Weight of Antigen

94kDa

Specificity & Comments

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Key References

  1. Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).
  2. Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

Bioinformatics

  •  Entrez Gene ID: 7184
  •  Gene Symbol: HSP90B1
  •  SwissProt: P14625
  •  Chromosome Location: 12q23.3
  •  Unigene: 192374
NBT03168