Immunofluorescence Analysis of HePG2 cells labeling GRP94 with GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot.(Red).

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GRP94 Monoclonal Antibody (SPM249).

Flow Cytometric Analysis of PFA-fixed HePG2 cells using GRP94 Monoclonal Antibody (SPM249) followed by Goat anti-Rat- IgG-CF488 (Blue); Isotype Control (Red).

Printable Datasheet

GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) [SPM249]

Name: GRP94 / HSP90B1 (Endoplasmic Reticulum Marker)
SKU: 7184-RTM1X
Availability: InStock

Monoclonals Primary Antibodies Rat Monoclonals

Summary

Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Product Properties & Targets

Host

Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Chicken, Cow, Dog, Guinea Pig, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus Laevis

Research Areas

Cardiovascular, Hypoxia

Isotype

IgG2a

Cellular Localization

Endoplasmic reticulum lumen, Melanosome, Sarcoplasmic reticulum lumen

Light Chain

Kappa

Gene Name

HSP90B1

Positive Control

HePG2. U20S cells. About 50% of breast tumors express grp94 protein.

Immunogen

Purified glucose regulated protein 94 (grp94) from chicken oviducts

Alternate Names

Endoplasmin, 94 kDa glucose-regulated protein, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog, Glucose Regulated Protein 94; 94kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

Database Links

Entrez Gene ID

7184

Unigene

192374

SwissProt

P14625

Additional Information

Clone

SPM249

Human Gene Symbol

HSP90B1

Chromosome Location

12q23.3

Mol. Weight of Antigen

94kDa

Functions

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059).

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Sorger, P.K. et al. J. Mol. Biol. 194: 341-344 (1987).
Tandon, A.K. et.al. Breast Cancer Res. and Treat. 16: 146 (1990).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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