Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R).
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using GATA3/4550R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of MCF-7 cell lysate using GATA-3 Recombinant Rabbit Monoclonal Antibody (GATA3/4550R). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).
Known Applications & Suggested Dilutions
Host / Ig Isotype
Mol. Weight of Antigen
Specificity & Comments
GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.