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Recombinant GATA-3 (Breast and Urothelial Marker) Antibody [rGATA3/3870]

Additional information

Catalog Number: 2625-MSM10
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2625-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2625-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2625-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant GATA-3 (Breast and Urothelial Marker) Antibody

Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant GATA-3 (Breast and Urothelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human urothelial carcinoma stained with GATA-3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870).

GATA3 Antibody in Western Blot (WB)

GATA3 Antibody in Western Blot (WB)

Western blot analysis of Jurkat cell lysate using GATA3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Clone
rGATA3/3870
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
GATA3
Positive Control
Human breast or urothelial carcinoma tissue. Jurkat.
Immunogen
Recombinant full-length human GATA-3 protein
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity).
Key References
  • Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.
  • Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2625
SwissProt
P23771
Chromosome Location
10p14
Mol. Weight of Antigen
~50kDa
Alternate Names
Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3; GATA binding protein-3; GATA-binding factor 3; GATA3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Summary

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

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