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GATA-3 (Breast and Urothelial Marker) Antibody [GATA3/2442]

Additional information

Catalog Number: 2625-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2625-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2625-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2625-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
GATA-3 (Breast and Urothelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).

GATA-3 (Breast and Urothelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GATA-3 Mouse Monoclonal Antibody (GATA3/2442).

GATA-3 (Breast and Urothelial Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified GATA-3 Mouse Monoclonal Antibody (GATA3/2442). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
GATA3/2442
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
GATA3
Positive Control
MCF-7 or T47D cells. Breast or Bladder Carcinoma.
Immunogen
Recombinant fragment of human GATA3 protein (around aa 357-436) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity).
Key References
  • Higgins JP, et al. Am J Surg Pathol. 2007; 31:673-680.
  • Liu H, et al. Am J Clin Pathol. 2012; 138:57-64.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2625
SwissProt
P23771
Chromosome Location
10p14
Mol. Weight of Antigen
~50kDa
Alternate Names
Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA3; GATA binding protein-3; GATA-binding factor 3; GATA3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3

Summary

GATA-3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA-3 expression is primarily seen in breast and urothelial carcinomas.’Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.

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