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FOXA1 / HNF3A

Mouse Monoclonal Antibody [Clone FOXA1/1519]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

Formalin-fixed, paraffin-embedded rat colon stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

Western blot analysis of human HeLa cell lysate using FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

SDS-PAGE Analysis Purified FOXA1 Mouse Monoclonal Antibody (FOXA1/1519). Confirmation of Integrity and Purity of Antibody.

Flow cytometric analysis of PFA-fixed MCF-7 cells using FOXA1 Mouse Monoclonal Antibody (FOXA1/1519) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescent analysis of PFA-fixed MCF-7 cells labeled with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519) followed by goat anti-mouse IgG-CF488. Counterstain: phalloidin.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

Formalin-fixed, paraffin-embedded rat colon stained with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

Western blot analysis of human HeLa cell lysate using FOXA1 Mouse Monoclonal Antibody (FOXA1/1519).

SDS-PAGE Analysis Purified FOXA1 Mouse Monoclonal Antibody (FOXA1/1519). Confirmation of Integrity and Purity of Antibody.

Flow cytometric analysis of PFA-fixed MCF-7 cells using FOXA1 Mouse Monoclonal Antibody (FOXA1/1519) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescent analysis of PFA-fixed MCF-7 cells labeled with FOXA1 Mouse Monoclonal Antibody (FOXA1/1519) followed by goat anti-mouse IgG-CF488. Counterstain: phalloidin.

Product Details

Synonyms

Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A)

Positive Control

HepG-2 or MCF-7 cells. Human liver, pancreas, bladder, prostate, colon or breast.

Known Applications & Suggested Dilutions

Flow Cyt, IF, Western Blot, IHC-P More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human FOXA1 protein (aa372-472) (exact sequence is proprietary)

Cellular Localization

Nuclear

Species Reactivity

Human and Rat.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

49kDa

Specificity & Comments

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 ( and ), HNF-3 (, and ), HNF-4 ( and ), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.