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Home >> Antibodies >> Fibronectin (8th Type III Repeat)

Fibronectin (8th Type III Repeat)

Mouse Monoclonal Antibody [Clone 568]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).

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SDS-PAGE Analysis Purified Fibronectin Monoclonal Antibody (568). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

Positive Control

SW156 cells. Kidney.

Known Applications & Suggested Dilutions

Flow Cyt, IF, IHC-P         More Details

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts

Cellular Localization

Connective tissue matrix

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

220kDa (monomer); 440kDa (dimer)

Specificity & Comments

Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Christensen, L., et al., APMIS, suppl. 26, 1-39 (1992).
  2. Christensen, L., et al., APMIS, 98(7), 615-623 (1990).
  3. Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473.

Bioinformatics

  •  Entrez Gene ID: 2335
  •  Gene Symbol: FN1
  •  SwissProt: P02751
  •  Chromosome Location: 2q35
  •  Unigene: 203717
NBT00351