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  • HuProt™ Validated

Fibronectin Antibody [FN1/3569]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 2335-MSM19
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2335-MSM19-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2335-MSM19-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2335-MSM19-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Fibronectin Antibody

IHC analysis of formalin-fixed, paraffin-embedded human lung. Staining using FN1/3569 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Fibronectin Antibody - Image 2

Formalin-fixed, paraffin-embedded human lung stained with Fibronectin Mouse Monoclonal Antibody (FN1/3569).

Fibronectin Antibody - Image 3

Formalin-fixed, paraffin-embedded human lung stained with Fibronectin Mouse Monoclonal Antibody (FN1/3569).

Fibronectin Antibody in Western Blot (WB)

Fibronectin Antibody in Western Blot (WB)

Western Blot Analysis of Human Placenta tissue lysate using Fibronectin Mouse Monoclonal Antibody (FN1/3569).

Fn1 Antibody in SDS-PAGE

Fn1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Fibronectin Mouse Monoclonal Antibody (FN1/3569). Confirmation of Purity and Integrity of Antibody.

Fibronectin Antibody in Western Blot (WB)

Fibronectin Antibody in Western Blot (WB)

Western blot analysis of human liver, lung and skeletal muscle tissue lysates using Fibronectin Mouse Monoclonal Antibody (FN1/3569).

  • ELISA
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FN1/3569
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
Fn1
Positive Control
SW156 cells. Human kidney, Placenta
Immunogen
Recombinant fragment (around aa 467-595) of human fibronectin protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
  • Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Key References
  • Ugarovaet. al. Biochemistry, 1996; 35:10913-10921.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2335
SwissProt
P02751
Chromosome Location
2q35
Mol. Weight of Antigen
220kDa (monomer); 440kDa (dimer)
Alternate Names
Fibronectin, Cold-insoluble globulin, Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

Summary

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

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