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Fibronectin (8th Type III Repeat) Antibody [568]

Additional information

Catalog Number: 2335-MSM4
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2335-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2335-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2335-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Fibronectin (8th Type III Repeat) Antibody

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).

Fibronectin (8th Type III Repeat) Antibody - Image 2

SDS-PAGE Analysis of Purified Fibronectin Monoclonal Antibody (568). Confirmation of Purity and Integrity of Antibody.

Fibronectin Antibody in Western Blot (WB)

Fibronectin Antibody in Western Blot (WB)

Western Blot Analysis of Human Placenta tissue lysate using Fibronectin Mouse Monoclonal Antibody (568).

Fibronectin Antibody in Western Blot (WB)

Fibronectin Antibody in Western Blot (WB)

Western Blot Analysis of Human Placenta tissue lysate using Fibronectin Mouse Monoclonal Antibody (568).

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
568
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
Fn1
Positive Control
SW156 cells. Human Kidney, Human Placenta
Immunogen
High molecular weight proteins secreted by cultivated human fibroblasts
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
  • Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
Key References
  • Christensen, L., et al., APMIS, suppl. 26, 1-39 (1992).
  • Christensen, L., et al., APMIS, 98(7), 615-623 (1990).
  • Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2335
SwissProt
P02751
Chromosome Location
2q35
Mol. Weight of Antigen
220kDa (monomer); 440kDa (dimer)
Alternate Names
Fibronectin, Cold-insoluble globulin, Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

Summary

Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

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