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Home >> Antibodies >> Fascin-1(Reed-Sternberg CellMarker)

Fascin-1(Reed-Sternberg CellMarker)

Recombinant Mouse Monoclonal Antibody [Clone rFSCN1/6464]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified Fascin-1 Recombinant Mouse Monoclonal Antibody (rFSCN1/6464). Confirmation of Integrity and Purity of Antibody.

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IHC analysis of formalin-fixed, paraffin-embedded human pancreas. Cytoplasmic staining using rFSCN1/6464 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

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IHC analysis of formalin-fixed, paraffin-embedded human kidney. Cytoplasmic staining using rFSCN1/6464 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Product Details

Synonyms

55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); SNL

Positive Control

HeLa, MCF-7, PC-3 or BEWO cells. Hodgkin s lymphoma, ovarian or testicular carcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Full length recombinant human FSCN1 protein

Cellular Localization

Cytoplasm

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Yamashiro-Matsumura S and Matsumura F. J Biol Chem 1985; 260(8): 5087.
  2. Yamashro-Matsumura S and Matsumura F. J Cell Biol 1986; 103:631.

Bioinformatics

  •  Entrez Gene ID: 6624
  •  Gene Symbol: FSCN1
  •  SwissProt: Q16658
  •  Chromosome Location: 7p22.1
  •  Unigene: 118400