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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)
Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)
SDS-PAGE Analysis Purified Fascin-1 Mouse Monoclonal Antibody (FSCN1/417). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of HeLa cells labeling Fascin with Fascin-1 Mouse Monoclonal Antibody (FSCN1/417)-CF640 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red) .
Flow Cytometric Analysis of PFA-fixed K562 cells using Fascin-1 Mouse Monoclonal Antibody (FSCN1/417) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
Western blot analysis of HeLa cell lysate using FSCN1 Mouse Monoclonal Antibody (FSCN1/417).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin’s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.
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