Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Fascin-1 (Reed-Sternberg Cell Marker) Antibody [FSCN1/7209]

In Stock
Catalog Number Formulation Size Price
6624-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6624-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6624-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell cortex, Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Cytosol, Filopodium, Invadopodium, Microvillus, Stress fiber
Gene Name
Positive Control
HeLa, MCF-7, Ovarian or Testicular Carcinoma., PC-3 or BEWO cells. Hodgkin s lymphoma
Immunogen
Recombinant fragment (around aa350-400) of human FSCN1 protein (exact sequence is proprietary)
Alternate Names
Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55, 55kDa actin-bundling protein; FAN1; Fascin homolog 1; Fascin1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); SNL

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
FSCN1/7209
Chromosome Location
7p22.1
Mol. Weight of Antigen
55kDa

Functions

  • Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945). Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786).

Key References

  • Yamashiro-Matsumura S and Matsumura F. J Biol Chem 1985; 260(8): 5087.
  • Yamashro-Matsumura S and Matsumura F. J Cell Biol 1986; 103:631.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Fascin-1 (Reed-Sternberg Cell Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK