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Home >> Antibodies >> ECD/ SGT1

ECD/ SGT1

MouseMonoclonal Antibody [Clone PCRP-ECD-1D10]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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SDS-PAGE Analysis Purified ECD Mouse Monoclonal Antibody (PCRP-ECD-1D10). Confirmation of Purity and Integrity of Antibody.

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Immunofluorescence analysis of PFA-fixed HeLa cells. ECD Mouse Monoclonal Antibody (PCRP-ECD-1D10) followed by goat anti-mouse IgG-CF488 (green).

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Flow cytometric analysis of PFA-fixed HeLa cells. ECD Mouse Monoclonal Antibody (PCRP-ECD-1D10) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using ECD Mouse Monoclonal Antibody (PCRP-ECD-1D10).

Product Details

Synonyms

Ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; SGT1 protein

Positive Control

HeLa or 293T cells.Human testis, spleen or colon.

Known Applications & Suggested Dilutions

IP, Flow Cyt, IF         More Details

Immunoprecipitation (1-2ug per 100-500ug of total protein)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant full-length human ECD protein

Cellular Localization

Nucleus. Cytosol.

Species Reactivity

Human. Predicted to react with Rabbit, Goat, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Orangutan and Elephant(based on sequence homology).

Host / Ig Isotype

Mouse/ IgG2b

Mol. Weight of Antigen

72.76kDa

Specificity & Comments

Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Sutcliffe EG, et al. Cancer Genet, 2020 Aug. PMID 32805687

Bioinformatics

  •  Entrez Gene ID: 11319
  •  Gene Symbol: ECD
  •  SwissProt: O95905
  •  Chromosome Location: 10q22.2
  •  Unigene: 631822