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ECD/ SGT1 Antibody [PCRP-ECD-1D10]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
11319-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
11319-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
11319-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml

Summary

Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes. Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins. The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG2b /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
ECD
Positive Control
HeLa or 293T cells.Human testis, spleen or colon.
Immunogen
Recombinant full-length human ECD protein
Alternate Names
Protein ecdysoneless homolog, Human suppressor of GCR two, Ecdysoneless homolog; hSGT1; Human suppressor of GCR two; Protein ecdysoneless homolog; SGT1 protein

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PCRP-ECD-1D10
Chromosome Location
10q22.2
Mol. Weight of Antigen
72.76kDa

Functions

  • Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212).

Key References

  • Sutcliffe EG, et al. Cancer Genet, 2020 Aug. PMID 32805687

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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