Home >> Antibodies >> Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)

Cytokeratin 3 (KRT3) (Corneal Epithelial Marker)

Mouse Monoclonal Antibody [Clone KRT3/2542]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Product Details

Synonyms

65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3

Positive Control

A431 cells. Human cornea.

Known Applications & Suggested Dilutions

Western Blot, IHC-P More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.

Cellular Localization

Cytoplasmic

Species Reactivity

Human, rabbit and bovine.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

65kDa

Specificity & Comments

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.