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Cytokeratin 3 (KRT3) (Corneal Epithelial Marker) Antibody [KRT3/2542]

In Stock
Catalog Number Formulation Size Price
3850-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3850-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3850-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis. Purified KRT3 Mouse Monoclonal Antibody (KRT3/2542). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis. Purified KRT3 Mouse Monoclonal Antibody (KRT3/2542). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins, which are arranged in pairs of heterotypic keratin chains co-expressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy.KRT3/2542 recognizes the 64kD polypeptide/keratin K3 (cytokeratin 3) of rabbit corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Bovine, Human, Rabbit
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT3
Positive Control
A431 cells. Human cornea.
Immunogen
Tissue, cells or virus corresponding to Rabbit Cytokeratin 3/CK-3. Rabbit corneal epithelial keratin.
Alternate Names
Keratin, type II cytoskeletal 3, 65 kDa cytokeratin, Cytokeratin-3, Keratin-3, Type-II keratin Kb3, 65 KDa Cytokeratin, CK3, CK-3, cytokeratin-3, Keratin, keratin 3, Keratin, Type II Cytoskeletal 3, Keratin-3, Type II Cytoskeletal 3, Type-II keratin Kb3

Database Links

Entrez Gene ID
38550
SwissProt
P12035

Additional Information

Clone
KRT3/2542
Chromosome Location
12q13.13
Mol. Weight of Antigen
65kDa

Related Antibodies

View All KRT3 Antibodies

Key References

  • Hanson C.,et al. Acta Ophthalmol.2013 Mar;91(2):127-30.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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