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Home >> Antibodies >> Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)

Recombinant Mouse Monoclonal Antibody [Clone rKRT20/6536]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
img

Formalin-fixed, paraffin-embedded human colon adenocarcinoma stained with Cytokeratin 20 Recombinant Mouse Monoclonal Antibody (rKRT20/6536). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

img

SDS-PAGE Analysis Purified KRT20 Recombinant Mouse Monoclonal Antibody (rKRT20/6536). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20

Positive Control

HT29 cells. Human normal colon or colon adenocarcinoma.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

Cellular Localization

Cytoplasm.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

46kDa

Specificity & Comments

This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Moll, R., et al. 1993. Differentiation 53: 75-93.
  2. Wauters, C.C., et al. 1995. Hum. Pathol. 26: 852-855.

Bioinformatics

  •  Entrez Gene ID: 54474
  •  Gene Symbol: KRT20
  •  SwissProt: P35900
  •  Chromosome Location: 17q21.2
  •  Unigene: 84905