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Home >> Antibodies >> CELA3B / ELA3B (Pancreatic Function Marker)

CELA3B / ELA3B (Pancreatic Function Marker)

Recombinant Mouse Monoclonal Antibody [Clone rCELA3B/1811]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
459.00
459.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
459.00
459.00
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SDS-PAGE Analysis Purified CELA3B Recombinant Mouse Monoclonal Antibody (rCELA3B/1811). Confirmation of Purity and Integrity of Antibody.

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Formalin-fixed, paraffin-embedded human pancreas stained with CELA3B Recombinant Mouse Monoclonal Antibody (rCELA3B/1811)

Product Details

Synonyms

Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E

Positive Control

Panc-28 or PANC1 cells. Human Pancreas.

Known Applications & Suggested Dilutions

Western Blot, IHC-P         More Details

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

~17kDa

Specificity & Comments

This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Tani, T., et al. 1988. J. Biol. Chem. 263: 1231-1239.
  2. Shimada, S., et al. 2002. Int. J. Mol. Med. 10: 599-603.

Bioinformatics

  •  Entrez Gene ID: 23436
  •  Gene Symbol: CELA3B
  •  SwissProt: P08861
  •  Chromosome Location: 1p36.12
  •  Unigene: 728752
NBT03435