CELA3B / ELA3B (Pancreatic Function Marker) [CELA3B/1374]

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Summary

This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Product Properties & Targets

Host
Species Reactivity
Research Areas
Isotype
Gene Name
CELA3B
Mol. Weight of Antigen
17kDa
Immunogen
Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Database Links

Entrez Gene ID
Unigene
SwissProt

Additional Information

Clone
CELA3B/1374
Human Gene Symbol
CELA3B
Chromosome Location
1p36.12

Known Applications & Suggested Dilutions

  • Western Blot (1-2ug/ml)
  • ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)Optimal dilution for a specific application should be determined.

Key References

  • Shirasu, Y., et al. 1988. Molecular cloning of complementary DNA encoding one of the human pancreatic protease E isozymes. J. Biochem. 104:259-264.
  • Tani, T., et al. 1988. Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning. J. Biol. Chem. 263: 1231-1239.
  • Shimada, S., et al. 2002. Pancreatic elastase IIIA and its variants are expressed in pancreatic carcinoma cells. Int. J. Mol. Med. 10: 599-603.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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