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Recombinant CELA3B / ELA3B (Pancreatic Function Marker) Antibody [rCELA3B/1811]

In Stock
Catalog Number Formulation Size Price
23436-MSM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
23436-MSM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
23436-MSM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This MAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.

Product Properties & Targets

Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Gene Name
Positive Control
Panc-28 or PANC1 cells. Human Pancreas.
Immunogen
Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
Alternate Names
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
rCELA3B/1811
Chromosome Location
1p36.12
Mol. Weight of Antigen
17kDa

Functions

  • Efficient protease with alanine specificity but only little elastolytic activity.

Key References

  • Tani, T., et al. 1988. J. Biol. Chem. 263: 1231-1239.
  • Shimada, S., et al. 2002. Int. J. Mol. Med. 10: 599-603.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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