Home >> Antibodies >> CD80 (B7-1)

CD80 (B7-1)

Mouse Monoclonal Antibody [Clone C80/3544]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human tonsil stained with CD80 Mouse Monoclonal Antibody (C80/3544).

Formalin-fixed, paraffin-embedded human tonsil stained with CD80 Mouse Monoclonal Antibody (C80/3544).

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD80 Mouse Monoclonal Antibody (C80/3544)

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human tonsil stained with CD80 Mouse Monoclonal Antibody (C80/3544).

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD80 Mouse Monoclonal Antibody (C80/3544)

Product Details

Synonyms

Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; Costimulatory factor CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80

Positive Control

Rajior Ramos cells. Human tonsil, spleen or thymus.

Known Applications & Suggested Dilutions

ELISA, IHC-P More Details

ELISA (For coating, order Ab without BSA)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment of human CD80 protein (around aa 35-142) (exact sequence is proprietary)

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

60kDa

Specificity & Comments

T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.