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CD80 (B7-1) Antibody [C80/3544]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
941-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
941-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
941-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Membrane
Gene Name
Positive Control
Rajior Ramos cells. Human tonsil, spleen or thymus.
Immunogen
Recombinant fragment of human CD80 protein (around aa 35-142) (exact sequence is proprietary)
Alternate Names
T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B7.1, Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80; Costimulatory factor CD80; CTLA-4 counter-receptor B7.1; LAB7; T-lymphocyte activation antigen CD80

Database Links

Entrez Gene ID
941
SwissProt

Additional Information

Clone
C80/3544
Chromosome Location
3q13.3-q21
Mol. Weight of Antigen
60kDa

Functions

  • Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
  • (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Key References

  • Peach, R.J., et al. 1995. J. Biol. Chem. 270: 21181-21187.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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