Home >> Antibodies >> CD56 / NCAM1 / NKH1 (Neuronal Cell Marker)

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker)

Mouse Monoclonal Antibody [Clone 123A8; same 56C04]

Printable

Key References

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Key References

Bioinformatics

Formalin-fixed, paraffin-embedded human thyroid stained with CD56 Mouse Monoclonal Antibody (123A8). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human pancreas stained with CD56 Mouse Monoclonal Antibody (123A8). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with CD56 Mouse Monoclonal Antibody (123A8).

SDS-PAGE Analysis Purified CD56 Mouse Monoclonal Antibody (123A8). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human brain tissue lysate using CD56 Mouse Monoclonal Antibody (123A8).

Formalin-fixed, paraffin-embedded human thyroid stained with CD56 Mouse Monoclonal Antibody (123A8). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human pancreas stained with CD56 Mouse Monoclonal Antibody (123A8). HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human colon stained with CD56 Mouse Monoclonal Antibody (123A8).

SDS-PAGE Analysis Purified CD56 Mouse Monoclonal Antibody (123A8). Confirmation of Purity and Integrity of Antibody.

Western blot analysis of human brain tissue lysate using CD56 Mouse Monoclonal Antibody (123A8).

Product Details

Synonyms

NCAM, Leu-19, NKH1, MSK39, NCAM120, NCAM140, NCAM180, Neural Cell Adhesion Molecule

Positive Control

Human cerebellum, pancreas or neuroblastoma.

Known Applications & Suggested Dilutions

IF, Western Blot, IHC-P More Details

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Membrane preparation of a small cell lung carcinoma

Cellular Localization

Cell surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

180, 145 and 125kDa

Specificity & Comments

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.