Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R).

SDS-PAGE Analysis Purified CD56 Rabbit Recombinant Monoclonal Antibody (NCAM1/2217R). Confirmation of Integrity and Purity of Antibody.

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD56 monoclonal antibody (NCAM1/2217R) (green).

CD56 / NCAM1 / NKH1 (Neuronal Cell Marker) [NCAM1/2217R]

Name: CD56 / NCAM1 / NKH1 (Neuronal Cell Marker)
SKU: 4684-RBM7
Availability: InStock

Monoclonals Primary Antibodies Rabbit Monoclonals Recombinant Rabbit Monoclonals Recombinants

Summary

This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Printable

Product Properties & Targets

Host

Rabbit

Species Reactivity

Human

Research Areas

Autophagy, Cardiovascular, Neuroscience

Isotype

IgG

Cellular Localization

Cell membrane, Secreted

Gene Name

NCAM1

Mol. Weight of Antigen

145 and 125kDa, 180

Immunogen

Recombinant full-length human NCAM1 (CD56) protein

Database Links

Entrez Gene ID

4684

Unigene

503878

SwissProt

P13591; P13592

Additional Information

Clone

NCAM1/2217R

Human Gene Symbol

NCAM1

Chromosome Location

11q23.1

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Hilkens et al., Int J Cancer. 1984 34:197-206.
Kibbelaar et al. Eur. J. Cancer 1991 27:431-435.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.