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Home >> Antibodies >> CD53 (TSPAN25)

CD53 (TSPAN25)

Mouse Monoclonal Antibody [Clone 161-2]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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SDS-PAGE Analysis Purified CD53 Mouse Monoclonal Antibody (161-2). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25

Positive Control

Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Known Applications & Suggested Dilutions

Func., Flow Cyt, IF         More Details

Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Immunogen

Stimulated human leukocytes

Cellular Localization

Cell Surface

Species Reactivity

Human. Does not react with Baboon or Horse.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

33-55kDa

Specificity & Comments

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Maecker HT, et al. The tetraspanin superfamily: molecular facilitators. FASEB J 1997, 11:428-442.
  2. Kishimoto T. et al., eds. Leukocyte Typing VI, p517-519 and p1153, Garland Publishing, Inc, New York and London, 1997.
  3. Rasmussen AM, et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 1994, 153:4997-5007.
  4. Szollosi J. et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 1996 Oct 1;157(7):2939-2946.
  5. Cao L. et al. Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation Immunobiology 1997, 197:70-81.

Bioinformatics

  •  Entrez Gene ID: 963
  •  Gene Symbol: CD53
  •  SwissProt: P19397
  •  Chromosome Location: 1p13
  •  Unigene: 443057
NBT00324