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CD53 (TSPAN25) Antibody [161-2]

Additional information

Catalog Number: 963-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
963-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
963-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
963-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD53 (TSPAN25) Antibody

SDS-PAGE Analysis of Purified CD53 Mouse Monoclonal Antibody (161-2). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • Functional Studies
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Clone
161-2
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Membrane
Gene Name
CD53
Positive Control
Daudi, HL-60, human leukocytes. Human tonsil and lymph node., HUT-102, HUT-78, IM9, Jurkat, Kg1a, Raji, THP-1, U266, YT
Immunogen
Stimulated human leukocytes
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).
Key References
  • Maecker HT, et al. The tetraspanin superfamily: molecular facilitators. FASEB J 1997, 11:428-442.
  • Kishimoto T. et al., eds. Leukocyte Typing VI, p517-519 and p1153, Garland Publishing, Inc, New York and London, 1997.
  • Rasmussen AM, et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 1994, 153:4997-5007.
  • Szollosi J. et al. Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY. J Immunol 1996 Oct 1;157(7):2939-2946.
  • Cao L. et al. Anti-CD53 monoclonal antibody induced LFA-1/ICAM-1-dependent and -independent lymphocyte homotypic cell aggregation Immunobiology 1997, 197:70-81.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
963
SwissProt
P19397
Chromosome Location
1p13
Mol. Weight of Antigen
33-55kDa
Alternate Names
Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25

Summary

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

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