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CD53 (TSPAN25) Antibody [63-5A3]

Additional information

Catalog Number: 963-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
963-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
963-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
963-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD53 (TSPAN25) Antibody

SDS-PAGE Analysis of Purified CD53 Mouse Monoclonal Antibody (63-5A3). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • Functional Studies
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Clone
63-5A3
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Membrane
Gene Name
CD53
Positive Control
Daudi, Raji and Jurkat cells. Human leukocytes. Human tonsil and lymph node.
Immunogen
Human Sezary cells
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Required for efficient formation of myofibers in regenerating muscle at the level of cell fusion. May be involved in growth regulation in hematopoietic cells (By similarity).
Key References
  • Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995.
  • Kishimoto T et al. eds. Leukocyte Typing VI, Garland Publishing, New York, 1997.
  • Knapp, W. et al., Leucocyte typing IV, p 534 and p 541. Oxford Univ. Press. 1989
  • Olweus J et al. CD53, a protein with four membrane-spanning domains, mediates signal transduction in human monocytes and B cells. J Immunol 1993, 151(2):707-716.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
963
SwissProt
P19397
Chromosome Location
1p13
Mol. Weight of Antigen
33-55kDa
Alternate Names
Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25); TSPAN25

Summary

Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.

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