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Home >> Antibodies >> CD5 (Mantle Cell Lymphoma Marker)

CD5 (Mantle Cell Lymphoma Marker)

Recombinant Mouse Monoclonal Antibody [Clone rC5/6462]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

219.00
499.00
499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00
499.00
499.00
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Formalin-fixed, paraffin-embedded human tonsil stained with CD5 Recombinant Mouse Monoclonal Antibody (rC5/6462).

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Formalin-fixed, paraffin-embedded human tonsil stained with CD5 Recombinant Mouse Monoclonal Antibody (rC5/6462).

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IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rC5/6462 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

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SDS-PAGE Analysis Purified CD5 Recombinant Mouse Monoclonal Antibody (rC5/6462). Confirmation of Purity and Integrity of Antibody.

Product Details

Synonyms

CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5

Positive Control

293T, Ramos, or MOLT-4 cells. Human tonsil or lymph node.

Known Applications & Suggested Dilutions

IHC-P         More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa450-495) of human CD5 protein.

Cellular Localization

Cell Surface

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2a, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10% of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20% of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Related Products



200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Ferry JA et. al. American Journal of Clinical Pathology, 1996, 105(1):31-7.
  2. Gagneten D et. al. Diagnostic Cytopathology, 1996, 14(1):32-7.

Bioinformatics

  •  Entrez Gene ID: 921
  •  Gene Symbol: CD5
  •  SwissProt: P06127
  •  Chromosome Location: 11q12.2
  •  Unigene: 58685