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CD4 (T-Helper/Inducer Cell Marker)

Mouse Monoclonal Antibody [Clone CD4/7144]

Printable

Bioinformatics

Catalog #

Size

20 ug

100 ug

Price (USD)

219.00

499.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Performance Guarantee

Catalog No

Size

20 ug

100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml

Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

219.00

499.00

Printable

Bioinformatics

SDS-PAGE Analysis Purified CD4 Mouse Monoclonal Antibody (CD4/7144). Confirmation of Purity and Integrity of Antibody.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Cell surface staining using CD4/7144 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Cell surface staining using CD4/7144 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

SDS-PAGE Analysis Purified CD4 Mouse Monoclonal Antibody (CD4/7144). Confirmation of Purity and Integrity of Antibody.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Cell surface staining using CD4/7144 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Cell surface staining using CD4/7144 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.

Product Details

Synonyms

L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Positive Control

Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60. Lymph node or tonsil.

Known Applications & Suggested Dilutions

IHC-P More Details

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant fragment (around aa200-400) of human CD4 protein (exact sequence is proprietary)

Cellular Localization

Cell surface.

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG2b, kappa

Mol. Weight of Antigen

55kDa

Specificity & Comments

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.