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Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody [rCD4/3930]

In Stock
Catalog Number Formulation Size Price
920-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
920-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
920-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCD4/3930 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human tonsil stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 4

SDS-PAGE Analysis Purified CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). Confirmation of Integrity and Purity of Antibody.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 5

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

CD4 Antibody in Western Blot (WB)

CD4 Antibody in Western Blot (WB)

Western Blot Analysis of human tonsil tissue lysate using CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4+/CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, AKT Signaling, Cytokine Signaling, Hematopoietic Stem Cells, Infectious Disease
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell membrane
Gene Name
CD4
Positive Control
CCRF-CEM or HL-60. Human lymph node or tonsil., Human peripheral blood mononuclear cells (PBMCs)
Immunogen
Recombinant fragment (around aa 200-300) of human CD4 protein (exact sequence is proprietary)
Alternate Names
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Database Links

Entrez Gene ID
920
SwissProt
P01730

Additional Information

Clone
rCD4/3930
Chromosome Location
12p13.31
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CD4 Antibodies

Functions

  • Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
  • (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:2214026, PubMed:16331979, PubMed:9641677, PubMed:12089508). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).

Key References

  • Garcia-Herrera A, et al. Primary cutaneous small/medium CD4+ T-cell lymphomas: a heterogeneous group of tumors with different clinicopathologic features and outcome. J Clin Oncol. 2008; 26:3364-71.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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